Page 69 - ESHRE2019

Zinc Sparks in OvstmCherry Rescue* but not OvstNull Eggs
MII Egg #1
OvstNull
OvstmCherry
Fluozin-3
(Zinc indicator)
MII Egg #2
OvstNull
MII Egg #3
OvstNull
40
OvstmCherry Rescue* Zinc
600 1200 1800 2400 3000 3600 4200 4800 5400
OvstNull
Does Zinc Functionally Affect the Zona Matrix? Pre-incubation of OvstNull Egg
Metal (50 uM)
MgSO4 ZnSO4
Sperm in the PVS
63.8 ± 4.0 61.2 ± 4.7
1. Pre-incubate egg with metal (1 hr)
2. Transfer to fresh media
3. Incubate with 3x105 ml-1 sperm (6 hr)
Injection into the Perivitelline Space of OvstNull Eggs
Metal (2 mM)
MgSO4 ZnSO4
Sperm in the PVS
64.0 ± 3.1 67.0 ± 2.7
1. Incubate egg after injection (1 hr)
2. Transfer to fresh media
3. Incubate with 3x105 ml-1 sperm (6 hr)
64
30
mCherry
20
10
40
30
20
10
40
35 30
25 20
Fluorescence (AU)
15 10
0
5 0
‐5 0 40
35 30
25 20
15 10
0
0
600 1200 1800 2400 3000 3600 4200 4800 5400 6000
5 0
‐5
0 12 24 36 48 60 Time (sec x 10-2)
*(proxy for WT)
PRECONGRESS COURSE 02 I VIENNA, AUSTRIA – 23 JUNE 2019 67