Page 68 - ESHRE2019

Zona Penetration in OvstNull and Zp2Mut Embryos
50 40 30 20 10
0 MII Eggs
2-Cell 4-Cell
• OvstNull – no block to zona penetration • absent in MII eggs
• absent in 2- and 4-cell embryos
• Zp2Mut – transient block to zona penetration • intact in MII eggs
• absent in 2- and 4-cell embryos
Zona Block is Rapid and Transient (suggests a small molecule)
“Zinc sparks are triggered by fertilization.......”
Teresa Woodruff & Tom O’Halloran
Kim, AM, et al., ACS Chem Biol 6:716, 2011 Que, EL et al., Nat Chem 7:130, 2015
66
63
Sperm in PVS/Egg