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OvstmCherry Mice Rescue OvstNull Phenotype
OvstmCherry
E1 AltE1E2 E3 E4E5E6
E7E8
mCherry
E9 Sperm binding
ZP2 cleavage
WT Null Resc E 2C E 2C E 2C
Anti-ZP2
OvstNull 2C WTEgg WT2-Cell OvstResc2C
kD 250
150
100 75
50 37
25
120 kD (uncleaved) 90 kD (cleaved)
Egg
2-Cell Embryos
PLOS Genetics 13:e1006580 2017
Rapid Block to Penetration
In vitro Fertilization
Hours after Insemination 123456
75
Anti-ZP2
Egg Activation with SrCl2
Minutes
0 1 5 10 20 30
Anti-ZP2
kD 150
ZP2Intact ZP2Cleaved
ZP2Intact ZP2Cleaved
Perivitelline Space (PVS)
Egg
kD 150
100 75
64
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