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SrCl2 activation (Zp2Mut MII eggs)
Add sperm 1-3 hr after egg activation
Transient Block to Sperm Penetration
6 hr
observe
Perivitelline Space
Delayed Insemination
1 hr 2 hr 3 hr
Transient Block
Block to Penetration is Absent in OvstNull and Zp2Mut Embryos Re-insemination
IVF of Zp2Mut or OvstNull eggs
Green tail sperm
6h 2-cell embryos
Re-insemination
6h
4-cell embryos
observe
observe
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PRECONGRESS COURSE 02 I VIENNA, AUSTRIA – 23 JUNE 2019 65
