Page 22 - PCC02
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   Glutamine beneficial, but chemically unstable ): NH4 accumulation
Protein supplements contain Glutamine
  NH4 accumulation at 37oC of various protein supplements
 Kleijkers et al. 2016a
  Time since production of culture media has an effect on phenotype
 • Culture media will chemically decompose
– Shelf life determined as the maximum time since production the culture media may be used
– Based in general on chemical analysis and biological assays (MEA)
• A large study (1832 IVF/ICSI cycles) found an association between birth weight and age of the culture media
  Kleijkers et al. 2015b
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