Page 23 - PCC02

Supplements to ART media
• It is necessary to supplement the basal salt solutions to:
– Facilitate fertilization and embryo culture. • Energy substrates, amino acids, ....
• Protein supplements...
– Compensate for ”adverse” in vitro conditions. • Antibiotics, pH regulators, osmolytes, surfactants...
ART Culture media Antibiotics
• Semen is not free from microorganisms
– Primarily bacteria
• Follicular aspirates often contaminated with vaginal flora
– Bacteria, yeast
• In vitro culture without antibiotics often will lead to growth of microorganisms
• Antibiotics used in ART
• Penicillin
– Prevent growth of bacteria
• Bacterial wall synthesis blocked • Streptomycin
– Kills bacteria
• r‐RNA inhibitor
• Gentamycin
– Kills bacteria
• r‐RNA inhibitor
• Most preparations of antibiotics are not specifically made for ART
– Historically been a problem
Cotell et al. 2000
Page 17 of 151
PRECONGRESS COURSE 02 I BARCELONA, SPAIN – 1 JULY 2018 23