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ART culture media naturally occurring additives
• Vitamins
– B’s and C
• Nucleotides • Steroids
– Estradiol, Progesterone, Cholesterol
• Hyaluronic acid
• Unclear effect/necessary? – B’s for DNA methylation?
• Unclear effect/necessary?
• Unclear effect/necessary?
• Positive effect in transfer media(?)
ART culture media
in vitro specific additives
• HEPES
• Phenol red
• Antioxidants – Taurine
– Ascorbic acid
• Surfactants
– Polyethylene glycol (PEG)
– Methylcellulose
– Pluronic‐F68
• pH regulator
– Buffer HCO3 containing media media in low
CO2
• pH‐indicator
– Comfort blanket ‐ not needed
• Neutralize oxygen radicals
– Reactive oxygen species (ROS) in semen
– Culture embryos in low O2 ( 5%)
• Regulate surface tension
– Handling small droplets
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