Page 20 - PCC02

Amino acid concentration
in vivo (tube) and in vitro (Eagle´s medium)
0.7 0.6 0.5 0.4 0.3 0.2 0.1
0
AGASGAGTATTMVPILL SLSELRLHLYREAHLEY PUNRNGYRARPTLEEUS
Amino acid
In vivo In vitro
Glycine
Outside Inside
Glutamine
Inhibited by energy substrates
and other amino acids
Amino acids
Amino acids
Amino acids compete for transport
Tay et al., 1997
Glycine
Glutamine
What happens to the amino acids in the medium?
Oxidised in the Krebs cycle
Energy (ATP) + CO2 + H20 + NH4+
Amino acids
Proteins
20
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mM