Page 19 - PCC02

• Improved blastocyst culture
• Feeder cells:
– secretes beneficial
molecules?
– maydetoxifyculture media?
• Concerns over biological safety was an issue
Co‐culture
Embryos cultured together with “feeder‐cells”
Feeder cells
Chinese Hamster Ovary cells
(CHO) VERO‐cells
Tubal epithelium Follicular cells
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Too little...or... too much
• What is the optimal concentration of the various ingredients in the medium?
– Absent (not needed) – Low
– Medium
– High
• Averycomplexdiscussionsinceeachingredientis not an independent variable
– A lot of interactions
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PRECONGRESS COURSE 02 I BARCELONA, SPAIN – 1 JULY 2018 19