Page 13 - PCC02

In the beginning
• Edwardsetal:
– simple salt solutions for
• oocyte,sperm,and fertilization
• Earle’s solution
– And complex culture media for embryo culture
• Ham F10
• Proteinsupplement added
• Essentially sequential culture media in the early days
Mice as models
Steptoe & Edwards, 1978 Elder & Johnson, 2015
• Mouseembryosare readily available and can be used as a model for systematic development of embryo culture media
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PRECONGRESS COURSE 02 I BARCELONA, SPAIN – 1 JULY 2018 13