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Optimization of IVF culture media
• The systematic approach:
• Simplexoptimization:
– Defined mouse strains
– Four ingredient in the media
• NaCl,KH2PO4,Glucose,Pyruvate
– Endpoint: Embryo development
Optimization of IVF culture media KSOM
15 media differences
6 trials
2 biological replicates 12 zygotes per replicate
): 2160 zygotes needed
Lawitt and Biggers , 1991
Ingredients
KSOM (mM)
NaCL
95
KCl
2,5
CaCl2
1,7
MgSO4x7H2O
0,2
NaHCO3
25
Na‐lactate
10
Na Pyruvate
0,2
KH2PO4
0,35
D‐Glucose
0,2
L‐Glutamine
1
• This approach lead to the development of KSOM and subsequently to some single‐step media
• Currentmedia
• + Amino acids
• + Protein supplement
• + Antibiotics
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