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Physiological salts
• Simple salt solutions:
– Earle’s basal salt solution (EBSS)
– Protein and antibiotics added for embryo culture
• Still the basis for some commercially available culture media
Continuous culture of mammalian cells
• Culture media for continuous culture of mammalian cells was developed in the 50‐ties and 60‐ties
– Must contains ”everything” a cell need to grow and survive in long term culture
– Examples
• Ham’sF10/F12
• Eagles’sMEM, • M199
– Was used as basis for some commercially available culture media
• StillusedinsomeapplicationslikeIVM
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