Page 11 - PCC02

Mouse embryo culture Simplex optimization
Different starting points
Basal salt solutions
Tubal fluid
Mammalian cell culture
ART Culture
media
Uterine fluid
Embryo metabolism
L-Amino acid -keto glutarate Ammonium NH3+ O O ion
R-CH-C-O- -O-C-CH2-CH2-C-C-O- NH4+ O O NADH
Transamination Oxidative deamination
O O NH3+ R-C-C-O- -O-C-CH2-CH2-CH-C-O-
OO -keto acid Glutamic acid
NAD+ + H2O
Physiological salts
Ingredients
Earle’s BSS (mM)
Hanks’s BSS (mM)
NaCL
114,4
137,0
KCl
5,4
5,4
CaCl2
0,2
1,3
MgSO4x7H2O
0,8
0,4
NaHCO3
26,2
4,2
NaH3PO4.H2O
0,8
‐
NaH3PO4. 7H2O
0,3
KH2PO4
‐
0,4
D‐Glucose
5,6
5,6
HEPES Na salt
50,0
8,0
• Simplesaltsolutions: – most prevalent ions
osmolality – pH
• closetophysiological conditions
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PRECONGRESS COURSE 02 I BARCELONA, SPAIN – 1 JULY 2018 11