Page 15 - PCC02

Mice and men
• It is not straight forward to extrapolate from animal models to humans
– Animal models, primarily using mice, have formed much of our understanding of early embryo physiology and the effects of culture media composition.
• But
– Hugedifferencesbetweendifferentmicestrains
• Which strain is most relevant for human IVF..?
– Andmicearenotmen..
• And vice versa I’ve been told.....
Back to Nature ?
• The in vivo conditions are the best (?)
• Should we try to emulate the conditions in the fallopian tube/uterus as best as we can (?)
– We must anyway compensate for factors that inevitable follows when culturing in vitro
• ”Culture media”
– Salts, energy, amino acids...
• Gas phase – O2, CO2
• pH
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PRECONGRESS COURSE 02 I BARCELONA, SPAIN – 1 JULY 2018 15