Page 166 - PRECONGRESS COURSE 01

Sperm preparation
Samples with a viral load ‐ HIV
Meta analysis
no randomized controlled trials – 12079 cycles
• Density gradient extended semen wash & swim up
• Some studies with ‘insert system’
• Check by PCR
• Semen RNA positivity after preparation: 1.3% to 7.7% ‐ samples not used
• Semen washing significantly reduced risk of transmission in HIV‐discordant couples regardless of viral suppression in the male partner
• No transmission to mother or infant was noted ESHRE, 2017 Sperm preparation, Nijs M.
Sperm preparation
Quality management
• All specimen containers, processing tubes and pipettes must be labeled according to local guidelines (EU tissue directive 2003)
• Use SEC if donor gametes are being processed (EU Directive 2015/565)
• Only CE labelled products/media that are intended for use in ART can be
used.
• No animal derived products can be used
• All materials and media batches should be traceable and hence traceable.
ESHRE, 2017 Sperm preparation, Nijs M.
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