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Sperm preparation
Media‐composition
• Presence of metals in discontinuous colloidal silicon gradients can exacerbate oxidative DNA damage (Aitken et al., 2014)
• Influence of sperm wash media composition on progressive motility and DNA fragmentation (Anbari et al., 2016)
• No influence on clinical outcomes in IVF is noted (Kim et al., 2014) ESHRE, 2017 Sperm preparation, Nijs M.
Few studies
Sperm preparation
Temperature
• T influences pH – also in buffered media
• T influences onset of capacitation
T Influence on Sperm parameters
• Progressive motility and morphology are significantly higher after incubation at RT compared with 35°C (Thijssen et al., 2014)
T influence on Fertilisation and/or Pregnancy rates? • Studies needed
ESHRE, 2017 Sperm preparation, Nijs M.
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