Page 163 - PRECONGRESS COURSE 01

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Sperm preparation
Media ‐ pH
The ideal sperm preparation medium helps maintain a stable pH and osmolality while providing the proper physiological environment for survival.
External pH of culture media (pHe) influences sperm binding and motility
Improper intracellular pH (pHi) can impede sperm motility and the acrosome reaction
Buffered media: Hepes, MOPS, combination with Sodium Bicarbonate No need for incubator – BUT pH depends onTemperature
Temperature °C
38 36 34 32 30 28 26 24 22 20 18 16 14 12 10
86420
7.58 7.56 7.55 7.54 7.53 7.52 7.5 7.44 7.48 7.47 7.46 7.44 7.43 7.42 7.41 7.4 7.38 7.3
ESHRE, 2017 Sperm preparation, Nijs M.
Sperm preparation
Media‐composition
• Bicarbonate stimulates adenylate cyclase in spermatozoa, and the bicarbonate‐sensitive adenylate cyclase system has been shown to regulate sperm motility
• Calcium is an essential bivalent cation for the acrosome reaction, and extracellular calcium is also required for the sperm capacitation process
• Proteins and magnesium are needed for chromatin stability in sperm, motility, and the acrosome reaction
• Albumin and HEPES are beneficial for the longer‐term storage
• Commercial media are available
ESHRE, 2017 Sperm preparation, Nijs M.
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PRECONGRESS COURSE 01 I GENEVA, SWITZERLAND – 2 JULY 2017 163