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Sperm preparation
Evidence for the Use of One technique Compared to Another
Multiple studies comparing Swim‐up vs Density gradient centrifugation
Recovery yield: Density gradient centrifugation is superior (WHO, 2010)
Sperm parameters: No consensus
• Motility
• Viability
• Total motile count
• Morphology
• DNA fragmentation
• Chromatin packaging
• ROS
ESHRE, 2017 Sperm preparation, Nijs M.
Sperm preparation
Evidence for the Use of One technique Compared to Another
Telemere length
• sperm telomere length is shorter in males who are infertile
• loss of telomere length results in the aging and apoptosis of male germ cells
• Sperm telomeres determine telomere length of early embryos and offspring
Swim‐up vs Density gradient centrifugation outcomes : First studies (Yang et al. 2015; Zhang et al.; 2016)
Spermatozoa with longer Telomeres lengths ↑ in favour of Density gradient centrifugation
ESHRE, 2017 Sperm preparation, Nijs M.
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