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Sperm preparation in the laboratory
Discontinuous density gradient centrifugation
Pros
• High recovery rate
• Clean fraction is obtained
• Density gradient will effectively remove
Lymphocytes, epithelial cells, abnormal, senescent and immature sperm, cell debris, seminal fluid, bacteria and to some extent viruses, sources of ROS, sperm with abnormal DNA
• The amount and volume of the layers can be adapted according to the initial sperm concentration, presenece of abnormal forms and motility (Nijs & Ombelet, 2003; Nijs, 2009) – very low sperm density samples can be processed
Cons
• Time consuming • More cost
• >45 minutes
ESHRE, 2017 Sperm preparation, Nijs M.
Sperm preparation
which method to choose?
Elephant spermatozoa
ESHRE, 2017 Sperm preparation, Nijs M.
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PRECONGRESS COURSE 01 I GENEVA, SWITZERLAND – 2 JULY 2017 157