Page 155 - PRECONGRESS COURSE 01

Sperm preparation in the laboratory
Three methods are routinely used
Simple dilution and washing technique Swim‐up technique
Discontinuous density gradient centrifugation
Sterile techniques should be used throughout specimen processing.
ESHRE, 2017 Sperm preparation, Nijs M.
Sperm preparation in the laboratory
Discontinuous density gradient centrifugation
• This method uses centrifugation of seminal plasma over density gradients consisting of colloidal silica coated with silane, which separates cells by their density.
• A simple two‐step discontinuous density‐gradient preparation method is prepared, typically with a 40% (v/v) layer over an 80% (v/v) density lower layer.
• Place 1 ml of semen above the density‐gradient media (More than one tube per semen sample may be used, if necessary.)
ESHRE, 2017 Sperm preparation, Nijs M.
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PRECONGRESS COURSE 01 I GENEVA, SWITZERLAND – 2 JULY 2017 155