Page 153 - PRECONGRESS COURSE 01

Sperm preparation in the laboratory
Three methods are routinely used
Simple dilution and washing technique Swim‐up technique
Discontinuous density gradient centrifugation
Sterile techniques should be used throughout specimen processing.
ESHRE, 2017 Sperm preparation, Nijs M.
Sperm preparation in the laboratory
Swim‐up technique
• Spermatozoa may be selected by their ability to swim out of seminal plasma and into culture medium. Hence “swim‐up” technique.
• The direct swim‐up technique can be performed either by layering culture medium (1,2 ml) over 1 ml of liquefied semen or by layering liquefied semen under the culture medium
• Incline the tube at an angle of about 45°, to increase the surface area of the semen–culture medium interface, and incubate for 1 hour at 37 °C.
ESHRE, 2017 Sperm preparation, Nijs M.
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PRECONGRESS COURSE 01 I GENEVA, SWITZERLAND – 2 JULY 2017 153