Page 152 - PRECONGRESS COURSE 01

Sperm preparation in the laboratory
Simple dilution and washing technique
• The sperm pellet is resuspended by gentle pipetting, in a volume of supplemented medium appropriate for final disposition, e.g. insemination
• This procedure can take up to 40 minutes.
ESHRE, 2017 Sperm preparation, Nijs M.
Sperm preparation in the laboratory
Simple dilution and washing technique
Pros
• Easy and Fast
• Cheap
• High yield Cons
• Pelleting of debris, dead and living spermatozoa, normal and abnormal spermatozoa, motile and immotile spermatozoa
• Presence of large numbers of non‐viable spermatozoa in the prepared sample ‐ can inhibit capacitation
• RS is present in the pellet oidave damage ESHRE, 2017 Sperm preparation, Nijs M.
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