Page 151 - PRECONGRESS COURSE 01

Sperm preparation in the laboratory
Three methods are routinely used
Simple dilution and washing technique
Swim‐up technique
Discontinuous density gradient centrifugation
Sterile techniques should be used throughout specimen processing.
ESHRE, 2017 Sperm preparation, Nijs M.
Sperm preparation in the laboratory
Simple dilution and washing technique
• This technique uses dilution and centrifugation.
• A wash solution containing antibiotics and protein
supplements is added to the ejaculate
• After repeated centrifugation (2‐3) at 300‐500g the seminal fluid is eliminated from the sample and the sperm cells are concentrated for insemination.
ESHRE, 2017 Sperm preparation, Nijs M.
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PRECONGRESS COURSE 01 I GENEVA, SWITZERLAND – 2 JULY 2017 151