Page 149 - PRECONGRESS COURSE 01

Sperm preparation
Capacitation
Capacitation is considered to be a coordinated series of events leading to a gradual destabilization of the spermatozoon that enables it to undergo the acrosome reaction and to penetrate the zona pellucida (Harrison, 1996).
From Leahy&Gadella, 2011
Capacitation of eutherian spermatozoa is essential for fertilization not only in vivo but also in vitro, and underlies the manipulation of spermatozoa for clinical in vitro fertilization (IVF).
Mortimor, Journal of Andrology, Vol. 21, No. 3, May/June 2000
ESHRE, 2017 Sperm preparation, Nijs M.
Sperm preparation in ART
• Testpreparation–‘capacitation’
• Intracervical Insemination ‐ ICI
• Intra Uterine Insemination ‐ IUI
• In vitro Fertilisation ‐ IVF
• Intra Cytoplasmic sperm Injection ‐ ICSI
• Cryopreservation of Sperm
ESHRE, 2017 Sperm preparation, Nijs M.
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