Page 54 - PCC08
P. 54
To investigate the transcriptome underlying differential implantation potential among aneuploid blastocysts
Individual Human Blastocyst
Significance performed with ANOVA in R
& Function Annotation using Ingenuity Pathway Analysis
RNA Isolation: PicoPure®
33‐54 millions reads Per sample Average =
44 million reads
cDNA Synthesis and Amplification: SMARTer® Ultra® Low RNA Kit
McCallie et al, 2016
Small Cell Number RNA‐seq: Illumina HiSEQ 4000 (aligned to the human genome)
Blastocyst Transcriptome Analysis
Aneuploid Blastocysts
Total # Differentially Expressed Genes
% Significantly
Decreased p‐Value
Genes
Trisomy 11 361
Monosomy 11 775
Trisomy 15 278
Monosomy 15 280
Page 48 of 102
83%
76%
80%
76%
<0.05*
<0.05*
<0.05*
<0.05*
> 2‐fold
McCallie et al, 2016
Monosomy 11 vs Trisomy 11
84% of the differentially expressed genes showed increased expression in Trisomy (p<0.05*)
Monosomy 15 vs Trisomy 15
68% of the differentially expressed genes showed increased expression in Trisomy (p<0.05*)
54
