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Nucleus
nuclear export
miR gene
Pol II/III
5’ DroshaDGCR8
Ibrahim, et al. BBA 2014
3’
AAA 3’ UTR AAA 3’ UTR
mRNA degradation
Cytoplasm RISC
Ribosome
Exportin 5
Pre-miR
Pre-miR
Dicer Cleavage
miR duplex
Helicase
5 3’
GpppG GpppG
AAA 3’ UTR AAA 3’ UTR
RISC
GpppG
Target mRNA
Pri-miR
GpppG
translational repression
low complementarity
high complementarity
’
Mature miR
Techniques for studying microRNA –diagnostics and analytics
Ibrahim SA et al. Biochim Biophys Acta. 2014 Aug;1840(8):2609-20.
– microRNA can be isolated from blood and patient tissue (including formalin-fixed samples) using modified standard RNA isolation procedures
– microRNA is biologically more stable towards degradation than mRNA (‚pre- degraded‘)
– microRNA can be isolated from samples which are critical for mRNA, e.g. formalin- fixed, paraffin-embedded tissues
– microRNA expression can be analysed by
-> microRNA profiling (different array formats and flow cytometric methods) -> real time RT-PCR (e.g. TaqMan-assays)
-> high througput sequencing
microRNAs in endometriosis | Prof. Dr. Martin Götte
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