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Quality and quantity of light impacting embryos in vitro ‐Time lapse wins ‐
Time lapse
Time lapse
Time lapse system wins over other microscope light sources
• No radiations from the “danger zone”
• Much lower total irradiation
• X1000 of total energy of Time lapse observation does not
affect mouse blastocyst development
Li et al., 2014
Temperature
• Impactoftemperatureontheoocytemeioticspindle and embryo metabolism is well‐known
• 37°C is commonly used and is based on the estimate of human core body temperature
• ... But in human and animal models, follicle and F. tubes temperatures are 1.5‐2.3°C lower than the rest of the body
• Human study does not confirm any benefit of a slightly lower incubation temperature
Hong et al., 2014
Tm°
MIIs
Fert rate
D3 cell no.
Blast rate
Usable blast rate
Aneuploidy rate
36°C
399
86.2
7.0 ± 0.1
51.6
41.2
42.5
37°C
406
82.0
7.7 ± 0.1
60.1
48.4
46.1
Page 81 of 151
PRECONGRESS COURSE 02 I BARCELONA, SPAIN – 1 JULY 2018 87
