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Individual or group culture ‐ Does it make difference? ‐
Group without direct contact, N=4 Embryos alter their surroundings
Individual ‐ isolated
Trimarchi et al., 2000
Embryos also secrete specific factors • Ubiquitin
• CRH
• ApoA1
Group, N=3
Group, N=4
Group culture in the bovine Optimal blastocyst formation rate occurs when
embryos are cultured 165 m apart Gopichandran and Leese, 2006
Also, ” Blastocyst cell number, pyruvate/glucose uptake and lactate production decrease as the inter‐embryo distance increases from 240 to 465 m ”
SIMILAR FINDINGS IN OTHER MODEL SPECIES
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PRECONGRESS COURSE 02 I BARCELONA, SPAIN – 1 JULY 2018 85