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Moessner and Dodson (1995)
Almagor et al. (1996)
Spyropoulou et al. (1999)
Rijnders and Jansen (1999)
Rebollar–Lazaro and Matson (2010)
Ebner et al.(2010)
Tao et al.(2013)
Group culture in human IVF ‐ Inconclusive data ‐
Paper
Group conditions
2–5 embryos per 1 ml
3–6 embryos per 700 μl
3–5 embryos per 20 μl
8–12 embryos (5 μl per embryo)
3–5 embryos per 15 μl
3–5 embryos per 30 μl
2–5 embryos per droplet 50 μl
Outcome
Significantly greater MCN from group culture, positive correlation between size of the co‐culture groups and cell number
Increased pregnancy and implantation rates from group culture
No statistical significance difference
No statistical significance difference
Similar pregnancy and implantation rates Group culture (up to Day 3) increased blastocysts utilized
Group culture superior for compaction and blastulation
Group culture of good embryos from day 3 significantly promoted blastocyst development
Wale and Gardner, 2015
Light
Variables
• Lightsource
- Invertedmicroscope - Stereo microscope
- Time‐lapse device
- Environment
• Time of exposure
• Target (cells and/or medium)
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