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pH regulation : Intracellular pH
Intracellular pH regulation is an important function necessary to maintain intracellular homeostasis Cells contain various mechanisms to regulate pHi,which vary for different cell types
MOST SENSİTİVE CELL STAGE!!
Denuded MII are incapable of actively regulating pHi Aneoploidy / meotic spindle damage???
Morula & Blastocysts Rigorous control over their pHi due to the formation of tight junctions between cells
• No optimal pH has been identified for human embryo culture
• Determination of the optimum pH for embryos is likely media dependent thus it’s advisable for each
lab. to do their own direct comparisons in respective culture systems
Sw ain RBM Online 2010 Sw ain HRU 2012
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PRECONGRESS COURSE 02 I BARCELONA, SPAIN – 1 JULY 2018 143
