Page 142 - PCC02

pH
• pH of culture media is very critical since it’s directly controlled by and can vary significantly among labs.
• It’s primarily determined by the bicarbonate concentration in the media and CO2 concentration of the culture incubators
• Although embryos can develop over a range of media pH, variations may impact embryo development
• pHi can impact embryo metabolism, development and fetal growth as shown in mammalian embryo models (Lane 2000, Squirrel 2001, Zender-Fox 2010)
pH regulation; Extracellular pH
Diffussion of CO2 into the media
Protein source &concentration
Elevation of the lab.
Embryo culture media pH 7.4 to 7.2
60% change H̟+
Swain et al., RBM 2010
Volume of media Surface area
Use of oil overlay Type of lid/dishware
̟
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