Page 133 - PCC02
P. 133
Wale & Gardner HRU 2016
Chemical and physical factors that might affect medium performance
1. CHEMICALFACTORS
• Concentration of O2 • Ammonium
• Volatile organics
2. PHYSICALFACTORS
• CO2- Temperature &pH- Oil overlay
• Incubator/incubation chamber/time-lapse • Pipetting induced shear stress
• Light
• Incubation volume/Embryo grouping
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PRECONGRESS COURSE 02 I BARCELONA, SPAIN – 1 JULY 2018 133
