Page 100 - Eshre 2018

Possible changer because...
Very efficient
WT allele restored in the vast majority of edited embryos (??) No off target mutations detected (safety)
Easy to carry out (it’s an ICSI!).
OCT4master transcription factor, important in early embryogenesis and pluripotency
The effects of knocking out OCT4 is different in mouse and human embryos:
in mouse embryos:
gene expression is not disrupted
the OCT4 ‐/‐ blastocyst is established, but cannot be maintained
in human 2PN embryos:
gene expression is disrupted
the OCT4 ‐/‐ blastocyst cannot be established
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