Page 102 - Eshre 2018
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High efficiency of targeting...
Early embryos (up to 8 cell stage): 8 cell stage up to blastocyst: 71% (5/8) of embryos edited 100% (8/8) of embryos edited
All embryos retained wt OCT in at least 1 cell All blastocysts retained a degree
of OCT4 expression
Conclusions...
OCT4 has different roles in mouse vs. human
KO of OCT4 affects gene expression in human but not mouse
CRISPR does not cause genomic instability or developmental arrest
This method allows direct study of the role of genes in preimplantation development.
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