Page 26 - PRECONGRESS COURSE 03

How does the SAC inhibit the APC?
Vacant Kinetochore
Aurora Mps1
Mad2 – Key component of SAC
Mps1 & Aurora Kinase – Upstream kinases
• Recruitment
• Amplify signal
–APC activator.
Mad2 Cdc20
Sequestered by SAC Cdc20
MCC
APC activity required for anaphase
Cdc20
Cdk1
Cyclin B1
A
A
P
PC
C
Inactive
Separase
Securin
• The checkpoint complex inhibits the APC
• Securin and Cyclin B1 are stable
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CHROMOSOME