Page 119 - PRECONGRESS COURSE 01

Clinical application of CASA / SFTs
 Retrospective analysis of a 9‐year dataset (2006–2014), 1959 cycles.  IVF used in 830 cycles, ICSI used in 1129 cycles (57.6%)
 Prevalence of low / no fertilization:
Fertilization
Low
Failed
Differences in prevalence analyzed using Fisher’s Exact Test
(MedCalc v13.1, www.medcalc.be)
 The risk of failed or poor fertilization at IVF was no different to that for ICSI, and actually trended lower.
IVF ICSI
16 34
(1.9%) (3.0%)
P=0.148 – 0.7%
IVF v ICSI –1.1%
14 27
(1.7%) (2.4%)
P=0.339
CFAS 61, Halifax, October 2015 © Oozoa Biomedical 2015 23
CASA for static image analysis
 Improved image acquisition capabilities and computing power → more applications for CASA
 Typically uses fixation and staining (attempting to use motile sperm requires the head, midpiece, and tail to be in the same focal plane)
 Examples:
 Morphology / morphometry
 DNA fragmentation: sperm chromatin dispersion assay  Vitality: e.g. Hamilton Thorne IDENT and VIADENT
 ARIC test (Acrosome Reaction to Ionophore Challenge)
ESHRE 2017: PCC01 © Oozoa Biomedical 2017 24
Page 115 of 175
PRECONGRESS COURSE 01 I GENEVA, SWITZERLAND – 2 JULY 2017 119