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Degrees of pluripotency
ENHSM 5iLAF NCM
Van der Jeught M et al. Hum Reprod Update. 2015 Sep‐Oct;21(5):616‐26
Culture conditions: effect of pluripotency state
Primed Naive Medium 1 Naive Medium 2 Naive Medium 3
Duggal et al. 2015 Gafni et al. 2013 Theunissen et al. 2014
• Stem cell heterogeneity upon conversion to naive conditions
• No specific naive conditions was associated with a higher rate of genomic instability
• Chromosomal instability cell line dependent
Abnormal samples
Early Passage
Intermediate Passage
UGENT11-60
Primed
0% (0/8)
0% (0/4)
Naive
17.4% (4/23)
37.5% (9/24)
UGENT11-2
Primed
0% (0/5)
0% (0/5)
Naive
8.7% (2/23)
12% (3/25)
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