Page 76 - ESHRE2019
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Culture conditions: oxygen tension
A comparison of chromosome abnormalities in hESCs at different passages
hESCs grown under normoxia and hypoxia at passage 63
Lim et al., Molecules and Cells, 2011
Culture conditions: culture density
• High culture density induces chromosomal instability
• Medium acidification linked to culture density main cause of DNA damage and chromosomal aberrations in hESCs
Jacobs et al., Stem Cell Reports, 2016
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