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Increased autophagy in aneuploid cells in the epiblast during pre-implantation and implantation
LC3B Misfolded protein Vesicle nucleation
Pre-implantation DAPI LC3B
Docking & fusion Lysosomal degradation During implantation
Protein fragments
EPI
Lumen
Developmental
Efficiency 100%
0.0Diploid Aneuploid Aneuploid BafA1
10 8 6 4 2 0
2.5 2.0 1.5 1.0 0.5 0.0
*
Diploid Aneuploid
Lysosome
Vesicle elongation
**
Diploid Aneuploid
Inhibition of autophagy prevents elimination of aneuploid cells
+/-Reversine Diploid
Lysosome
Aneuploid
25.5%
35
Bafilomycin A1 (BafA1)
+/- Autophagy inhibitor (BafA1) Aneuploid BafA1
91.8%
ns
DAPI
LC3B
2.0 1.6 1.2 0.8 0.4
*
Proportion of cells in EPI relative to diploids
Aneuploid Diploid
Number of LC3B puncta/cell in EPI
Aneuploid Diploid
Number of LC3B puncta/cell in EPI
