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p53-autophagy pathway eliminates aneuploid cells
Key
Diploid Control siRNA
Aneuploidy
Diploid p53 siRNA
Aneuploid Control siRNA
Control siRNA p53 siRNA
Apoptosis
cytosol nucleus
Misfolded & unfolded protein
Cell Survival
p53 siRNA/Control siRNA
+/-Reversine
Aneuploid p53 siRNA
Stress p53 pathway
LC3B DAPI
Number of LC3B puncta/cell in EPI
Chronic misfolding/ aggregation
Autophagy ensures aneuploid cells do not proceed through development beyond implantation in mouse epiblast
Diploid Cell
Aneuploid Cell
cytosol nucleus
Cell Death
Proteasome Protein Autophagy fragments
Native protein
Chromosome
mRNA
36
6 5 4 3 2 1 0
Autophagy
ns
*
Aneuploid
** ns
Diploid
l
PRECONGRESS COURSE 10 I VIENNA, AUSTRIA – 23 JUNE 2019 39
