Page 33 - ESHRE2019

Preferential elimination of aneuploid cells during pre-implantation stage via apoptosis
E2.0
E2.5
Diploid
Reversine Aneuploid
(8-cell) E2.5
mosaic chimera
(Early (Late Blastocyst) Blastocyst) E3.5 E4.5
Combined lineages ICM
TE
Diploid - Aneuploid
Time
8-cell
Early Late Blatocyst Blastocyst
Bolton H., et al. (2016)
Faster elimination of aneuploid cells when surrounded by diploid cells
Reversine Reversine
Diploid
Aneuploid
Aneuploid
Aneuploid
Aneuploid Diploid-Aneuploid
*
Time (hours) after E3.75
6 5 4 3 2 1 0
4 8 12 16 20 24
Diploid-aneuploid mosaics deplete aneuploid cells faster than other mosaics
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PRECONGRESS COURSE 10 I VIENNA, AUSTRIA – 23 JUNE 2019 31
Number of aneuploid (red) cells undergoing apoptosis
H2B-GFP Tomato
% of Aneuploid cells within lineage