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Reversine treatment induces aneuploidy in mouse embryos
Reversine
Diploid
Aneuploid
Proportion of aneuploidy determines embryos survival and decreases with embryo development
Aneuploid
Transfer
1:3 diploid aneuploid
1:1 diploid aneuploid
Transfer E13.5
H2B GFP
Transfer E13.5
H2B GFP
100% lethal
Aneuploidy Decreases
Normal
Embryo Viability Increases
Diploid Reversine
Aneuploid
mosaic chimera
Diploid
Diploid
Diploid
(Fetal Biopsy)
H2B GFP DAPI
Diploid
Aneuploid
(Fetal Biopsy)
H2B GFP DAPI
Partial
development development development
Normal
27
Loss of aneuploid cells
in fetuses of mosaic chimeras
Bolton H., et al. (2016)
Bolton H., et al. (2016)
