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Model: Sperm Bind to N-terminus of ZP2 Zona
Pellucida
Sperm
• The N-terminus of ZP2 is the zona pellucida ligand for capacitated sperm binding
• Neither N- nor O-glycans on the N-terminus of ZP2 are essential for gamete recognition
Science 329:216, 2010 J Cell Biol 197:897, 2012 J Cell Biol 205:801, 2014
Science Transl Med 8:336ra60, 2016 Dev Cell 46:247, 2018
Mouse Transgenesis: Gain of Function
Human sperm bind to human, but not mouse zonae
Human Zona Pellucida Mouse Zona Pellucida
Cross huZP2Transgene into moZp2Null background Monoclonal antibodies to: Monoclonal antibodies to:
huZP1 huZP2 NormahluZP3 huZP4 moZP1 moZP2 moZP3 Egg
huZP2 Rescue
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PRECONGRESS COURSE 02 I VIENNA, AUSTRIA – 23 JUNE 2019 55
