Page 96 - Eshre 2018
P. 96
hiPSCs
DAPI
DAPI
Activin A + BMP4 + bFGF
hPGC precursors
BMP4 + LIF hPGCLCs hPGCLCs
SOX2
hiPSCs
NANOG
OCT4 MERGED
Bar,
100μm Bar, 100μm
MERGED
Bar, 100μm
SOX17 BLIMP1
OCT4
DAPI
MERGED
Bar, 200μm
NANOG
NANOS3
2
1 1 1
0.5 0.5 000
1.5
1.5
SOX2
hPGC Precursors
Brachyury
BLIMP1 OCT4
3000 2000 1000
10 5
300 200 100
T
BLIMP1
000
A, Activin A; B, BMP4; F, bFGF
Zhou et al.
Complete Meiosis from Embryonic Stem Cell-Derived Germ Cells In Vitro.
Cell Stem Cell 18, 1–11 (2016)
Ishikura et al.
In Vitro Derivation and Propagation of Spermatogonial Stem Cell Activity from Mouse Pluripotent Stem Cells. Cell Rep. 17:2789-2804 (2016)
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hiPSCs hPGC... hPGCL...
A+B+F No cytokines
E8 FTDA CM
BMP4+LIF No cytokines
