Page 111 - Eshre 2018

Temperature + 20 0C
– 40 0C
– 196 0C
Vitrification
High concentration cryoprotectants
Slow freezing
Water
Low concentration cryoprotectants
Water
Water Extracellular ice
Dead Viable
SLOW-FREEZING VERSUS VITRIFICATION OF OVARIAN TISSUE: WHICH IS THE BETTER?
 Isachenko V et al., 2009:
 During a 16 day culture period there were no difference in
oestradiol, progesterone secretion or follicle quality.
 The percentage of follicles with normal morphologically was similar in both groups after thawing.
 Keros V et al., 2009:
 Based on tissue from 20 women the study concluded that vitrification was comparable to slow freezing in terms of preserving follicles in human ovarian tissue
 However, ovarian stroma retained a better morphological integrity after vitrification.
Isachenko V et al., Reproduction, 2009 24
Viable
23
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