Page 112 - Eshre 2018

VITRIFICATION VERSUS SLOW FREEZING: A SYSTEMATIC REVIEW AND META-ANLAYSIS
Proportion of intact primordial follicles
Proportion of normal stromal cells
DNA fragmentation in primordial follicles
 Meta-analysis of 14 studies comparing vitrification with slow freezing for cryopreservation of human ovarian tissue (Shi Q et al., Sci Rep. 2017)
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VITRIFICATION APPEARS TO BE A LITTLE BETTER, HOWEVER, CLINICAL RESULTS ARE AWAITED
 Meta-analysis (Shi Q et a., 2017) conclude that vitrification may be more effective than slow freezing, with less primordial follicular DNA strand breaks and better preservation of stromal cells. These advantages could lead to improved ovarian function after transplantation.
 However, the primordial follicle is pretty resistant to freezing and well-preserved with both methods, and in the end it is the clinical data which will finally decide which technique will be superior – but this will take years, if not decades...
 Basically slow-freezing and vitrification do the same thing but at a different speed and at different temperatures - they can probably be used with almost equal efficacy.
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