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FREEZING OVARIAN TISSUE
IN DENMARK THE OVARIAN TISSUE IS FROZEN BY CONTROLLED SLOW-FREEZING
Freezing solution:
1.5 M Ethyleneglycol; 0.1 M Sucrose; 10 mg/ml HSA
Temperature profile:
1. Equilibration – rotation (1-2 0C for 30 min)
2. –20C/minuntil–90C
3. Manuel seeding
4. – 0,3 0C/min until – 40 0C
5. – 10 0C/min until – 140 0C
6. Liquid nitrogen (– 196 0C)
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PRECONGRESS COURSE 16 I BARCELONA, SPAIN – 1 JULY 2018 109
