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“Safety zone”
• Normal OPU‐ICSI interval is within the “safety zone”
• No point in changing the interval of 1‐2 hours within the times of normal practice
Pujol et al., 2018
Standard vs. short insemination time in conventional IVF (non‐ICSI)
• Routinely, oocytes are exposed to thousands of sperm for at least 16 h
• Not strictly required: sperm penetrate the cumulus cell vestment and ZP by 2 hours
• Potential factors affecting the oocyte/embryo
• ROS from dead/apoptotic sperm
• Detrimental paracrine factors released by decaying cumulus cells
• Alteration of culture conditions
• Possible alternative modality
• Coincubation of egg/sperm for 1‐5 hours
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