Page 82 - PCC02

Culture media
• Ammonium accumulation
• Derived mainly from breakdown of L‐glutamine
• Embryotoxic above 75 m
L‐glutamine not replaced by more stable alanyl‐glutamine or glycil‐glutamine
Storage at 2‐8°C Kleijkers et al., 2016
Use at 37°C
Cleavage media
Washing mineral oil reduces contaminants and embryotoxicity
Mineral oil can contain embryotoxic contaminants
Morbeck et al., 2010
Unwashed
HTF HTF‐HSA Washing agent
Water
82
Page 76 of 151
% blastocyst