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Global Gene expression profiles and culture conditions
• Prospective randomized study • (Dutch multicentre trial)
– Human tubal fluid (HTF) (Lonza)
– G5‐Plus (Vitrolife)
– 5% and 20% O2
• Gene expression modulated both by culture media and O2 concentration
• Culture media effect dependent on O2 concentration
• “Developmental stage (embryo)and maternal age had a larger effect on the global gene expression profile... than the culture medium or oxygen concentration...”
Mantikou et al. 2016
Birth weight all
3500
3450
3400
3350
3300
3250
3200
Z‐score birth weights
0.15 0.1 0.05 0 ‐0.05 ‐0.1 ‐0.15
Multicenter, prospective radomized double‐blind study
Kleijkers et al. 2016b
G5 HTF
Significantly different
G5 HTF
Significantly different
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